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biotinylated peanut agglutinin (pna)  (Vector Laboratories)


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    Vector Laboratories biotinylated peanut agglutinin (pna)
    Biotinylated Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+peanut+agglutinin/Biotinylated+Peanut+Agglutinin+(PNA)/custom%40b-1075%4042414656
    Average 95 stars, based on 912 article reviews
    biotinylated peanut agglutinin (pna) - by Bioz Stars, 2026-10
    95/100 stars

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    other:

    Article Title: Safety and efficacy of AAV8-aflibercept in treating choroidal neovascularization via single-cell RNA sequencing
    Article Snippet: They were observed under an Olympus DP74 microscope (Olympus, Germany).

    Article Title: Recombinant lubricins, and compositions and methods for using the same
    Article Snippet: SynMuc1 was desalted into PBS using a Zeba Spin Desalting Column (87766, Thermo).

    Incubation:

    Article Title: Safety and Efficacy of AAV8-aflibercept in Treating Choroidal Neovascularization via Single-Cell RNA Sequencing
    Article Snippet: They were observed under an 494 Olympus DP74 microscope (Olympus, Germany). .. Sections were blocked and incubated with primary antibodies 498 against Gadd45b (Thermo Fisher, PA5-43160, 1:200), Ctss (Bioss, bs-8558R, 1:100), 499 Rhodopsin (Abcam, ab5417, 1:500), and biotinylated peanut agglutinin (PNA; Vector 500 Laboratories, B-1075-5, 1:200) overnight at 4°C. .. Sections were blocked and incubated with primary antibodies 498 against Gadd45b (Thermo Fisher, PA5-43160, 1:200), Ctss (Bioss, bs-8558R, 1:100), 499 Rhodopsin (Abcam, ab5417, 1:500), and biotinylated peanut agglutinin (PNA; Vector 500 Laboratories, B-1075-5, 1:200) overnight at 4°C.

    Article Title: Gne deletion in adult mice can cause thrombocytopenia, anemia, myopathy, bleeding, and death.
    Article Snippet: .. Biotinylated peanut agglutinin (PNA, B-1075-5), erythrina cristagalli lectin (ECA, B-1145-5), sambucus nigra lectin (SNA, B-1305-2), and maackia amurensis lectin II (MAA, B-1265-1) (all from Vector Laboratories; Plain City, OH) were added to the appropriate muscle sections along with rat monoclonal anti-laminin 2, α2 chain (L0663; Sigma Aldrich; St Louis, MO) and incubated overnight at 4 °C. .. Cy3-conjugated streptavidin (Jackson ImmunoResearch; West Grove, PA) and AlexaFluor 647-conjugated anti-rat IgG (Invitrogen; Waltham, MA) were added for an hour at room temperature, then slides were coverslipped using ProLong Gold antifade mountant with DAPI (ThermoFisher Scientific; Waltham, MA) and protected from light until imaging.

    Article Title: Production of recombinant IgA1 with defined mucin-type O-glycans in Nicotiana tabacum BY-2 cells
    Article Snippet: For western blotting, the membranes were saturated into Tris Buffer Saline containing 0.5% Tween ® 20 (TBST) and 3% (w/v) milk powder, before incubation with rabbit polyclonal antibodies against hGalNAcT2 (Sino Biologicals, 13764-T62, 1:2,000) and anti-rabbit HRP polyclonal antibodies (SynAbs, LO-RG-1-HRP, 1:10,000), or goat polyclonal antibodies against human IgA (alpha heavy chain) couple to HRP (Invitrogen, 31417, 1:25,000). .. For lectin blotting, the PVDF membranes were saturated in TBST containing 2% (w/v) BSA before incubation with biotinylated Helix pomatia agglutinin (Sigma-Aldrich L6512, 1:2,500) or biotinylated peanut agglutinin (Vector Laboratories B1075, 1:2,500), followed by incubation with Streptavidin HRP (Roche 11089153001, 1:5,000). .. Chemiluminescence was detected after revelation with BM chemilumiscence blotting substrate (Roche 11500694001) using Amersham Imager 600 (GE Healthcare).

    Labeling:

    Article Title: Atp1b2 Atp1b1 Knock-In Mice Exhibit a Cone–Rod Dystrophy-Like Phenotype
    Article Snippet: The sections were blocked in PBS containing 0.1% bovine serum albumin (BSA; Sigma-Aldrich, Deisenhofen, Germany) and 0.3% Triton X-100, incubated with the primary antibodies , washed, and incubated with Cy2- or Cy3-conjugated secondary antibodies (1:200; Jackson Immunoresearch Laboratories, West Grove, PA, USA). .. Cone photoreceptor cells were also labeled using biotinylated peanut agglutinin (PNA; 1:5000; Vector Laboratories, Burlingame, CA, USA) and Cy3-conjugated streptavidin (1:500; Jackson Immunoresearch Laboratories). .. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich).

    Article Title: Atp1b2Atp1b1 Knock-In Mice Exhibit a Cone–Rod Dystrophy-Like Phenotype
    Article Snippet: The sections were blocked in PBS containing 0.1% bovine serum albumin (BSA; Sigma-Aldrich, Deisenhofen, Germany) and 0.3% Triton X-100, incubated with the primary antibodies (Table 1), washed, and incubated with Cy2- or Cy3-conjugated secondary antibodies (1:200; Jackson Immunoresearch Laboratories, West Grove, PA, USA). .. Cone photoreceptor cells were also labeled using biotinylated peanut agglutinin (PNA; 1:5000; Vector Laboratories, Burlingame, CA, USA) and Cy3-conjugated streptavidin (1:500; Jackson Immunoresearch Laboratories). .. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich).



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